Fig. 7. GA protects HDFs from UV-B induced DNA damage. GA protects the UV-B -irradiated HDFs from DNA damage as is clear from western blotting analysis of key DNA damage response proteins, pATM (Fig. 7A), pATR (Fig. 7B), pChk1 (Fig. 7C), pChk2 (Fig. 7D), DDB2 (7E), pP53 (Fig. 7F) & χH₂AX (Fig. 7G), (*p<0.05 as compared to untreated, (*p<0.05, **p<0.01 as compared to UV-B only). We found that GA pre-treatment significantly downregulated the expression of DNA damage marker proteins by imparting protection to cells against oxidative stress & ER stress-mediated cell death effect. The protection by GA from UV-B induced DNA damage was more prominent in 6 h post-treatment by about double fold at 50µM concentration.